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THE M2VP GSH SCAVENGER
M2VP accomplishes complete scavenging of GSH in less than one minute. All other methods systematically overestimate GSSG concentrations. Before you measure GSSG, be sure you have M2VP.
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BIOXYTECH® GSH/GSSG-412
Catalog Number: 21040

Colorimetric Determination of Reduced and Oxidized Glutathione

**For research use only. Not for use in diagnostic procedures.**

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Introduction:
Reduced glutathione (GSH), a tripeptide (g-glutamylcysteinylglycine) with a free thiol group, is a major antioxidant in human tissues that provides reducing equivalents for the glutathione peroxidase (GPx) catalyzed reduction of hydrogen peroxide and lipid hydroperoxides to water and the respective alcohol. During this process GSH becomes oxidized glutathione (GSSG). The GSSG is then recycled to GSH by reduction by the reduced form of beta-nicotinamide adenine dinucleotide phosphate (NADPH), catalyzed by glutathione reductase (GR). When mammalian cells are exposed to increased oxidative stress, the ratio of GSH/GSSG will decrease as a consequence of GSSG accumulation. The measure of the GSSG level, or determining the GSH/GSSG ratio, is a useful measure of oxidative stress and to monitor the effectiveness of antioxidant intervention strategies.



Assay Precision:

GSSG in Buffer

      

 Whole Blood

 

n=26

High

Low

GSH

GSSG

MeanRate at A412

0.4194

0.0427

0.2938

0.00490

Intra-assay (% CV)

0.59

3.50

0.96

6.45

Intra-assay (% CV)

2.84

2.96

3.11

7.61

Total Precision (% CV)

2.87

3.86

3.18

8.86



MSDS:
View MSDS for BIOXYTECH® GSH/GSSG-412